Repurposing Triphenylmethane Dyes to Bind to Trimers Derived from Aβ.

ABSTRACT: Soluble oligomers of the β-amyloid peptide, Aβ, are associated with the progression of Alzheimer's disease. Although many small molecules bind to these assemblies, the details of how these molecules interact with Aβ oligomers remain unknown. This paper reports that crystal violet, and other C3 symmetric triphenylmethane dyes, bind to C3 symmetric trimers derived from Aβ_17-36. Binding changes the color of the dyes from purple to blue, and causes them to fluoresce red when irradiated with green light. Job plot and analytical ultracentrifugation experiments reveal that two trimers complex with one dye molecule. Studies with several triphenylmethane dyes reveal that three N, N-dialkylamino substituents are required for complexation. Several mutant trimers, in which Phe₁₉,

SUBMITTER: Salveson PJ 

PROVIDER: S-EPMC6339561 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature