A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin.

ABSTRACT: For modern approaches in precision medicine, fast and easy-to-use point-of-care diagnostics (POCs) are essential. Digoxin was chosen as an example of a drug requiring close monitoring. Digoxin is a cardiac glycoside used for the treatment of tachycardia with a narrow therapeutic window of 0.5-2.0 ng·mL^-1, and toxic effects are common for concentrations above 2.5 ng·mL^-1. For monitoring of blood concentration levels and treatment of intoxication, highly selective antibodies for digoxin and its hapten, digoxigenin, are available. A smartphone readout system is described for measuring digoxigenin in human serum using a common gold nanoparticle lateral flow assay (LFA). The R-package GNSplex, which also includes a Shiny app for quantitative test interpretation based on li

SUBMITTER: Ruppert C 

PROVIDER: S-EPMC6339659 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature