Visualizing the Distribution of Matrix Metalloproteinases in Ischemic Brain Using In Vivo ¹⁹F-Magnetic Resonance Spectroscopic Imaging.

ABSTRACT: Matrix metalloproteinases (MMPs) damage the neurovascular unit, promote the blood-brain barrier (BBB) disruption following ischemic stroke, and play essential roles in hemorrhagic transformation (HT), which is one of the most severe side effects of thrombolytic therapy. However, no biomarkers have presently been identified that can be used to track changes in the distribution of MMPs in the brain. Here, we developed a new ¹⁹F-molecular ligand, TGF-019, for visualizing the distribution of MMPs in vivo using ¹⁹F-magnetic resonance spectroscopic imaging (¹⁹F-MRSI). We demonstrated TGF-019 has sufficient sensitivity for the specific MMPs suspected in evoking HT during ischemic stroke, i.e., MMP2, MMP9, and MMP3. We then utilized it to assess those MMPs at 22 to

SUBMITTER: Huber VJ 

PROVIDER: S-EPMC6339703 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature