Targeted substrate degradation by Kelch controls the actin cytoskeleton during ring canal expansion.

ABSTRACT: During Drosophila oogenesis, specialized actin-based structures called ring canals form and expand to accommodate growth of the oocyte. Previous work demonstrated that Kelch and Cullin 3 function together in a Cullin 3-RING ubiquitin ligase complex (CRL3^Kelch) to organize the ring canal cytoskeleton, presumably by targeting a substrate for proteolysis. Here, we use tandem affinity purification followed by mass spectrometry to identify HtsRC as the CRL3^Kelch ring canal substrate. CRISPR-mediated mutagenesis of HtsRC revealed its requirement in the recruitment of the ring canal F-actin cytoskeleton. We present genetic evidence consistent with HtsRC being the CRL3^Kelch substrate, as well as biochemical evidence indicating that HtsRC is ubiquitylated and

SUBMITTER: Hudson AM 

PROVIDER: S-EPMC6340150 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature