Differential miRNA Expression Profiles in Cumulus and Mural Granulosa Cells from Human Pre-ovulatory Follicles.

ABSTRACT:

Background

Mural Granulosa Cells (MGCs) and Cumulus Cells (CCs) are two specialized cell types that differentiate from a common progenitor during folliculogenesis. Although these two cell types have specialized functions and gene expression profiles, little is known about their microRNA (miRNA) expression patterns.

Objective

To describe the miRNA profile of mural and cumulus granulosa cells from human preovulatory follicles.

Methods

Using small RNA sequencing, we defined the miRNA expression profiles of human primary MGCs and CCs, isolated from healthy women undergoing ovum pick-up for in vitro Fertilization (IVF).

Results

Small RNA sequencing revealed the expression of several hundreds of miRNAs in MGCs and CCs with 53 miRNAs being significantly differentially

SUBMITTER: Andrei D 

PROVIDER: S-EPMC6340152 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature