Molecular Basis for Autocatalytic Backbone N-Methylation in RiPP Natural Product Biosynthesis.

ABSTRACT: N-methylation of nucleic acids, proteins, and peptides is a chemical modification with significant impact on biological regulation. Despite the simplicity of the structural change, N-methylation can influence diverse functions including epigenetics, protein complex formation, and microtubule stability. While there are limited examples of N-methylation of the α-amino group of bacterial and eukaryotic proteins, there are no examples of catalysts that carry out post-translation methylation of backbone amides in proteins or peptides. Recent studies have identified enzymes that catalyze backbone N-methylation on a peptide substrate, a reaction with little biochemical precedent, in a family of ribosomally synthesized natural products produced in basidiomycetes. Here, we describe the crystal stru

SUBMITTER: Ongpipattanakul C 

PROVIDER: S-EPMC6340302 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature