Dysregulation of human miRNAs and increased prevalence of HHV miRNAs in obese periodontitis subjects.

ABSTRACT:

Aim

To evaluate human and herpesvirus-encoded microRNA (miRNA) expression in healthy and diseased gingiva of obese and non-obese subjects and compare the impact of localized and systemic inflammation on human miRNA profiles.

Material and methods

Healthy and inflamed gingival biopsies were collected from obese and non-obese subjects. Human and herpesvirus miRNA expression was quantified using quantitative PCR. Predicted targets of dysregulated miRNAs were identified using bioinformatics analysis, validated by dual luciferase assays and their expression assessed in healthy and diseased tissues.

Results

Our results show differential expression of miRNAs in both diseased groups compared to healthy counterparts. MMP-16 is identified as a novel target of miRNAs altered in d

SUBMITTER: Naqvi AR 

PROVIDER: S-EPMC6340734 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature