Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage.

ABSTRACT: Tissue fibrosis is a major cause of mortality that results from the deposition of matrix proteins by an activated mesenchyme. Macrophages accumulate in fibrosis, but the role of specific subgroups in supporting fibrogenesis has not been investigated in vivo. Here, we used single-cell RNA sequencing (scRNA-seq) to characterize the heterogeneity of macrophages in bleomycin-induced lung fibrosis in mice. A novel computational framework for the annotation of scRNA-seq by reference to bulk transcriptomes (SingleR) enabled the subclustering of macrophages and revealed a disease-associated subgroup with a transitional gene expression profile intermediate between monocyte-derived and alveolar macrophages. These CX3CR1⁺SiglecF⁺ transitional macrophages localized to the fibroti

SUBMITTER: Aran D 

PROVIDER: S-EPMC6340744 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature