Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells.

ABSTRACT: Microorganisms adapt their biophysical properties in response to changes in their local environment. However, quantifying these changes at the single-cell level has only recently become possible, largely relying on fluorescent labeling strategies. In this work, we utilize yeast (Saccharomyces cerevisiae) to demonstrate label-free quantification of changes in both intracellular osmolarity and macromolecular concentration in response to changes in the local environment. By combining a digital holographic microscope with a millifluidic chip, the temporal response of cellular water flux was successfully isolated from the rate of production of higher molecular weight compounds, in addition to identifying the produced compounds in terms of the product of their refractive index increment [Formula

SUBMITTER: Midtvedt D 

PROVIDER: S-EPMC6341078 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature