Detection of plant protein in adulterated milk using nontargeted nano-high-performance liquid chromatography-tandem mass spectroscopy combined with principal component analysis.

ABSTRACT: The objective of this study was to detect plant protein adulterated in fluid milk using nano-high-performance liquid chromatography (HPLC)-tandem mass spectroscopy (LC-MS/MS) combined with proteomics. Unadulterated milk and samples adulterated with soy protein, pea protein, hydrolyzed wheat protein, and hydrolyzed rice protein were prepared, with plant protein level ranged from 0.5% to 8% in total protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels clearly revealed that centrifugation at 20,000 g for 60 min would reduce band intensity of casein and albumin in milk. Results of nano-HPLC-MS/MS indicated the major proteins of soy (β-conglycinin, glycinin), pea (vincilin, convicilin, legumin), and wheat (glutenin and gliadin) in adulterated milks, allowing

SUBMITTER: Yang J 

PROVIDER: S-EPMC6341172 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature