Establishment of an erythroid cell line from primary CD36+ erythroid progenitor cells.

ABSTRACT:

Objective

Most continuous cell lines with erythroid characteristics are derived from patients with myelogenous leukemia or erythroleukemia. Among them, a few cell lines have been reported to be positive for CD36. We tried to establish a continuous erythroid cell line from the primary CD36(+) erythroid progenitor cells (EPCs) by the lentivirus-mediated gene transduction system.

Materials and methods

A lentiviral vector carrying SV40T, hTERT, or the human papillomavirus type 16 (HPV16) E6 and E7 (E6/E7) viral oncogenes, was introduced into CD36(+) EPCs, singularly or combined. Transformed cells were characterized in terms of histology, phenotype, karyotype, and gene expression profile.

Results

The lentiviral vector carrying HPV16 E6/E7 genes successfully transformed CD3

SUBMITTER: Wong S 

PROVIDER: S-EPMC6341492 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature