Elevated expression of Gab1 promotes breast cancer metastasis by dissociating the PAR complex.

ABSTRACT:

Background

Breast cancer (BCa) remains as the second leading cause of cancer-related death in women worldwide. The majority of the deaths are due to its progression to metastatic BCa. Although Grb2-associated binding protein 1 (Gab1) has been implicated in tumor proliferation and metastasis in multiple tumors including colorectal cancer, hepatocellular carcinoma and ovarian cancer, whether and how it regulates BCa metastasis are still poorly understood.

Methods

Western blot assay and immunohistochemical (IHC) staining were performed to assess expression of Gab1 in primary and metastatic BCa clinical samples. Biological function assay studies in vitro and in vivo were employed to investigate the functions of Gab1 during BCa metastasis. Co-immunoprecipitation (co-IP) assessmen

SUBMITTER: Wang X 

PROVIDER: S-EPMC6341703 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature