CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance.

ABSTRACT:

Background

NPR1, nonexpressor of pathogenesis-related gene 1, is a master regulator involved in plant defense response to pathogens, and its regulatory mechanism in the defense pathway has been relatively clear. However, information about the function of NPR1 in plant response to abiotic stress is still limited. Tomato is the fourth most economically crop worldwide and also one of the best-characterized model plants employed in genetic studies. Because of the lack of a stable tomato NPR1 (SlNPR1) mutant, little is known about the function of SlNPR1 in tomato response to biotic and abiotic stresses.

Results

Here we isolated SlNPR1 from tomato 'Ailsa Craig' and generated slnpr1 mutants using the CRISPR/Cas9 system. Analysis of the cis-acting elements indicated that SlNPR1 migh

SUBMITTER: Li R 

PROVIDER: S-EPMC6341727 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature