Functional characterization and proteomic analysis of lolA in Xanthomonas campestris pv. campestris.

ABSTRACT:

Background

The gram-negative Xanthomonas campestris pv. campestris is the pathogenic bacterium that causes black rot disease in crucifers. The virulence determinants of this bacterium include extracellular enzymes, exopolysaccharides, and biofilm formation. Here, one transposon mutant of X. campestris pv. campestris strain 17 that affects biofilm formation was isolated, and subsequent analyses led to the identification of the lolA gene, which encodes an outer membrane lipoprotein chaperone.

Results

The lolA mutant exhibited significant reductions in bacterial attachment, extracellular enzyme production, virulence, and tolerance in the presence of myriad membrane-perturbing agents. These phenotypic changes of the mutant could be complemented to the wild-type level through the

SUBMITTER: Liao CT 

PROVIDER: S-EPMC6341742 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature