Characterization of neurite dystrophy after trauma by high speed structured illumination microscopy and lattice light sheet microscopy.

ABSTRACT:

Background

Unbiased screening studies have repeatedly identified actin-related proteins as one of the families of proteins most influenced by neurotrauma. Nevertheless, the status quo model of cytoskeletal reorganization after neurotrauma excludes actin and incorporates only changes in microtubules and intermediate filaments. Actin is excluded in part because it is difficult to image with conventional techniques. However, recent innovations in fluorescent microscopy provide an opportunity to image the actin cytoskeleton at super-resolution resolution in living cells. This study applied these innovations to an in vitro model of neurotrauma.

New method

New methods are introduced for traumatizing neurons before imaging them with high speed structured illumination microscopy or

SUBMITTER: Phillips JK 

PROVIDER: S-EPMC6342006 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature