Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining.

ABSTRACT:

Premise of the study

Ephedra sinica (Ephedraceae) is a gymnosperm shrub with a wide distribution across Central and Eastern Asia. It is widely cultivated as a medicinal plant, but its wild populations are monitored to determine whether protection is needed.

Methods and results

Thirty-six microsatellite markers, including 11 polymorphic markers, were developed from E. distachya RNA-Seq data deposited in the National Center for Biotechology Information dbEST database. Among 100 genotyped E. sinica individuals originating from five different population groups, the allele number ranged from three to 22 per locus. Levels of observed and expected heterozygosity ranged from 0 to 0.866 (average 0.176) and 0 to 0.876 (average 0.491), respectively. Allelic polymor

SUBMITTER: Jiao SQ 

PROVIDER: S-EPMC6342172 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature