Detection of Ligand-binding to Membrane Proteins by Capacitance Measurements.

ABSTRACT: In multi-cellular organisms, cells communicate with each other utilizing chemical messengers. For many of these messenger molecules, the membrane is an insurmountable barrier. Yet, they act by binding to surface proteins often triggering a cascade of reactions inside the cell. Accordingly, studying ligand-receptor interactions at the cellular surface is key to understanding important aspects of membrane biology. However, despite a multitude of approaches to study membrane features, there is a need for developing techniques that can measure ligand binding with high temporal resolution and on a single cellular level. We recently developed a label-free approach to study ligand binding in real time. This methodology capitalizes on changes of the membrane's surface potential induced by the adso

SUBMITTER: Burtscher V 

PROVIDER: S-EPMC6342252 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature