Glutathione exposes sequential IgE-epitopes in ovomucoid relevant in persistent egg allergy.

ABSTRACT:

Scope

Patients with persistent egg allergy have more immunoglobulin E (IgE) against sequential than conformational epitopes of ovomucoid (OVO). Here, we aimed to identify compounds capable to render sequential epitopes in egg.

Methods and results

Glutathione was used for in vitro reduction of OVO and circular dichroism analyses were performed. Glutathione reduced OVO in a concentration-dependent manner. Egg white was analyzed for reduced proteins with a thiol probe and by MALDI-TOF/TOF. In unprocessed total egg white, several reduced proteins were detected by the thiol probe, among them reduced ovalbumin could be confirmed with MS analyses. Egg-allergics or sensitized controls were tested serologically (n = 19) for IgE against native and reduced OVO and in skin prick tests (

SUBMITTER: Roth-Walter F 

PROVIDER: S-EPMC6342257 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature