Connexin43 and zonula occludens-1 are targets of Akt in cardiomyocytes that correlate with cardiac contractile dysfunction in Akt deficient hearts.

ABSTRACT: While deletion of Akt1 results in a smaller heart size and Akt2^-/- mice are mildly insulin resistant, Akt1^-/-/Akt2^-/- mice exhibit perinatal lethality, indicating a large degree of functional overlap between the isoforms of the serine/threonine kinase Akt. The present study aimed to determine the cooperative contribution of Akt1 and Akt2 on the structure and contractile function of adult hearts. To generate an inducible, cardiomyocyte-restricted Akt2 knockout (KO) model, Akt2^flox/flox mice were crossed with tamoxifen-inducible MerCreMer transgenic (MCM) mice and germline Akt1^-/- mice to generate the following genotypes:Akt1^+/+; Akt2^flox/flox (WT), Akt2^flox/flox; α-MHC-MCM (iAkt2 KO), Akt1^-/-,

SUBMITTER: Ock S 

PROVIDER: S-EPMC6342503 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature