RNA-seq for identification of therapeutically targetable determinants of immune activation in human glioblastoma.

ABSTRACT:

Introduction

We sought to determine which therapeutically targetable immune checkpoints, costimulatory signals, and other tumor microenvironment (TME) factors are independently associated with immune cytolytic activity (CYT), a gene expression signature of activated effector T cells, in human glioblastoma (GBM).

Methods

GlioVis was accessed for RNA-seq data from The Cancer Genome Atlas (TCGA). For subjects with treatment-naïve, primary GBM, we quantified mRNA expression of 28 therapeutically targetable TME factors. CYT (geometric mean of GZMA and PRF1 expression) was calculated for each tumor. Multiple linear regression was performed to determine the relationship between the dependent variable (CYT) and mRNA expression of each of the 28 factors. Variables associated with CYT

SUBMITTER: Bagley SJ 

PROVIDER: S-EPMC6342649 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature