Proteomics and bioinformatics analyses identify novel cellular roles outside mitochondrial function for human miro GTPases.

ABSTRACT: The human Miro GTPases (hMiros) have recently emerged as important mediators of mitochondrial transport and may significantly contribute to the development of disorders such as Alzheimer's and schizophrenia. The hMiros represent two highly atypical members of the Ras superfamily, and exhibit several unique features: the presence of a GTPase domain at both the N-terminus and C-terminus, the presence of two calcium-binding EF-hand domains and localisation to the mitochondrial outer membrane. Here, elucidation of Miro GTPase signalling pathway components was achieved through the use of molecular biology, cell culture techniques and proteomics. An investigation of this kind has not been performed previously; we hoped, through these techniques, to enable the profiling and identification of path

SUBMITTER: Kay LJ 

PROVIDER: S-EPMC6342832 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature