Development of a rapid profiling method for the analysis of polar analytes in urine using HILIC-MS and ion mobility enabled HILIC-MS.

ABSTRACT:

Introduction

As large scale metabolic phenotyping is increasingly employed in preclinical studies and in the investigation of human health and disease the current LC-MS/MS profiling methodologies adopted for large sample sets can result in lengthy analysis times, putting strain on available resources. As a result of these pressures rapid methods of untargeted analysis may have value where large numbers of samples require screening.

Objectives

To develop, characterise and evaluate a rapid UHP-HILIC-MS-based method for the analysis of polar metabolites in rat urine and then extend the capabilities of this approach by the addition of IMS to the system.

Methods

A rapid untargeted HILIC LC-MS/MS profiling method for the analysis of small polar molecules has been developed.

SUBMITTER: King AM 

PROVIDER: S-EPMC6342856 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature