Engineering Clostridial Aldehyde/Alcohol Dehydrogenase for Selective Butanol Production.

ABSTRACT: Butanol production by Clostridium acetobutylicum is accompanied by coproduction of acetone and ethanol, which reduces the yield of butanol and increases the production cost. Here, we report development of several clostridial aldehyde/alcohol dehydrogenase (AAD) variants showing increased butanol selectivity by a series of design and analysis procedures, including random mutagenesis, substrate specificity feature analysis, and structure-based butanol selectivity design. The butanol/ethanol ratios (B/E ratios) were dramatically increased to 17.47 and 15.91 g butanol/g ethanol for AAD_F716L and AAD_N655H, respectively, which are 5.8-fold and 5.3-fold higher than the ratios obtained with the wild-type AAD. The much-increased B/E ratio obtained was due to the dramatic

SUBMITTER: Cho C 

PROVIDER: S-EPMC6343042 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature