Monoclonal antibody against EV71 3D^pol inhibits the polymerase activity of RdRp and virus replication.

ABSTRACT:

Background

Enterovirus A 71 (EV71) is a neurotropic virus that may lead to acute flaccid paralysis, encephalitis, cardiopulmonary failure or even death. No vaccine and defensive drug controlling EV71 is currently available, novel and efficient antiviral drug or vaccine is therefore urgently needed. 3D^pol (RNA-dependent RNA polymerase (RdRp)) has been an important target for anti-EV71 drug development.

Methods

A panel of monoclonal IgG antibodies (mAbs) against EV71 3D^pol were generated by traditional cell fusion methods. And the antibody affinity and specificity to EV71 3D^pol were evaluated by Enzyme-linked Immunosorbent Assay (ELISA), Indirect Fluorescent Assay (IFA) and Western blotting. Antiviral activities of these antibodies were also de

SUBMITTER: Li Y 

PROVIDER: S-EPMC6343263 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature