The multi-functionality of N-809, a novel fusion protein encompassing anti-PD-L1 and the IL-15 superagonist fusion complex.

ABSTRACT: Here we describe a novel bifunctional fusion protein, designated N-809. This molecule comprises the IL-15/IL15Rα superagonist complex containing the Fc-domain of IgG1 (N-803, formerly designated as ALT-803) fused to two single chain anti-PD-L1 domains. The fully human IgG1 portion of the N-809 molecule was designed to potentially mediate antibody dependent cellular cytotoxicity (ADCC). The studies reported here show that N-809 has the same ability to bind PD-L1 as an anti-PD-L1 monoclonal antibody. RNAseq studies show the ability of N-809 to alter the expression of an array of genes of both CD4⁺ and CD8⁺ human T cells, and to enhance their proliferation; CD8⁺ T cells exposed to N-809 also have enhanced ability to lyse human tumor cells. An array of genes wa

SUBMITTER: Jochems C 

PROVIDER: S-EPMC6343815 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature