Blood transcriptomic markers of Mycobacterium tuberculosis load in sputum.

ABSTRACT:

Background

Peripheral blood transcriptome signatures that distinguish active pulmonary tuberculosis (TB) from control groups have been reported, but correlations of these signatures with sputum mycobacterial load are incompletely defined.

Methods

We assessed the performance of published TB transcriptomic signatures in Haiti, and identified transcriptomic biomarkers of TB bacterial load in sputum as measured by Xpert® MTB/RIF molecular testing. People in Port au Prince, Haiti, with untreated pulmonary TB (n = 51) formed the study cohort: 19 people with low and 32 with high sputum Mycobacterium tuberculosis load. Peripheral whole blood transcriptomes were generated using RNA sequencing.

Results

Twenty of the differentially expressed transcripts in TB vs. no TB were diff

SUBMITTER: Dupnik KM 

PROVIDER: S-EPMC6343854 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature