OsDCL1a activation impairs phytoalexin biosynthesis and compromises disease resistance in rice.

ABSTRACT:

Background and aims

MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional regulators of gene expression via sequence-specific cleavage or translational repression of target transcripts. They are transcribed as long single-stranded RNA precursors with unique stem-loop structures that are processed by a DICER-Like (DCL) ribonuclease, typically DCL1, to produce mature miRNAs. Although a plethora of miRNAs have been found to be regulated by pathogen infection in plants, the biological function of most miRNAs remains largely unknown. Here, the contribution of OsDCL1 to rice immunity was investigated.

Methods

Activation-tagged Osdcl1a (Osdcl1a-Ac) rice mutants were examined for resistance to pathogen infection. mRNA and small RNA deep sequencing, quantitati

SUBMITTER: Salvador-Guirao R 

PROVIDER: S-EPMC6344094 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature