A Versatile and Open-Source Rapid LED Switching System for One-Photon Imaging and Photo-Activation.

ABSTRACT: Combining fluorescence and transmitted light sources for microscopy is an invaluable method in cellular neuroscience to probe the molecular and cellular mechanisms of cells. This approach enables the targeted recording from fluorescent reporter protein expressing neurons or glial cells in brain slices and fluorescence-assisted electrophysiological recordings from subcellular structures. However, the existing tools to mix multiple light sources in one-photon microscopy are limited. Here, we present the development of several microcontroller devices that provide temporal and intensity control of light emitting diodes (LEDs) for computer controlled microscopy illumination. We interfaced one microcontroller with μManager for rapid and dynamic overlay of transmitted and fluorescent images. More

SUBMITTER: Battefeld A 

PROVIDER: S-EPMC6344383 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature