In vivo assessment of increased oxidation of branched-chain amino acids in glioblastoma.

ABSTRACT: Altered branched-chain amino acids (BCAAs) metabolism is a distinctive feature of various cancers and plays an important role in sustaining tumor proliferation and aggressiveness. Despite the therapeutic and diagnostic potentials, the role of BCAA metabolism in cancer and the activities of associated enzymes remain unclear. Due to its pivotal role in BCAA metabolism and rapid cellular transport, hyperpolarized ¹³C-labeled α-ketoisocaproate (KIC), the α-keto acid corresponding to leucine, can assess both BCAA aminotransferase (BCAT) and branched-chain α-keto acid dehydrogenase complex (BCKDC) activities via production of [1-¹³C]leucine or ¹³CO₂ (and thus H¹³CO₃^-), respectively. Here, we investigated BCAA metabolism

SUBMITTER: Suh EH 

PROVIDER: S-EPMC6344513 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature