Quorum sensing in Pseudomonas aeruginosa mediated by RhlR is regulated by a small RNA PhrD.

ABSTRACT: Pseudomonas aeruginosa is a highly invasive human pathogen in spite of the absence of classical host specific virulence factors. Virulence factors regulated by quorum sensing (QS) in P. aeruginosa cause acute infections to shift to chronic diseases. Several small regulatory RNAs (sRNAs) mediate fine-tuning of bacterial responses to environmental signals and regulate quorum sensing. In this study, we show that the quorum sensing regulator RhlR is positively influenced upon over expression of the Hfq dependent small RNA PhrD in Pseudomonas. RhlR transcripts starting from two of the four different promoters have same sequence predicted to base pair with PhrD. Over expression of PhrD increased RhlR transcript levels and production of the biosurfactant rhamnolipid and the redox active pyocyanin

SUBMITTER: Malgaonkar A 

PROVIDER: S-EPMC6344545 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature