Label-Free Optical Detection of DNA Translocations through Plasmonic Nanopores.

ABSTRACT: Solid-state nanopores are single-molecule sensors that hold great potential for rapid protein and nucleic-acid analysis. Despite their many opportunities, the conventional ionic current detection scheme that is at the heart of the sensor suffers inherent limitations. This scheme intrinsically couples signal strength to the driving voltage, requires the use of high-concentration electrolytes, suffers from capacitive noise, and impairs high-density sensor integration. Here, we propose a fundamentally different detection scheme based on the enhanced light transmission through a plasmonic nanopore. We demonstrate that translocations of single DNA molecules can be optically detected, without the need of any labeling, in the transmitted light intensity through an inverted-bowtie plasmonic nanopo

SUBMITTER: Verschueren DV 

PROVIDER: S-EPMC6344913 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature