Sox7 negatively regulates prostate-specific membrane antigen (PSMA) expression through PSMA-enhancer.

ABSTRACT:

Background

PSMA expression in the prostate epithelium is controlled by a cis-element, PSMA enhancer (PSME). PSME contains multiple binding sites for Sox proteins, and in this study, we identified Sox7 protein as a negative regulator of PSMA expression through its interaction with PSME.

Methods

The statistical correlation between Sox7 and PSMA mRNA expression was evaluated using five prostate cancer studies from cBioportal. In vitro and in vivo interaction between Sox7 and PSME was evaluated by chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA), and luciferase reporter assay. Synthetic oligonucleotides were generated to define the sites in PSME that interact with Sox7 protein. Sox7 mutants were generated to identify the region of this protein re

SUBMITTER: Peng W 

PROVIDER: S-EPMC6344945 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature