Anoctamin Channels in Human Myometrium: A Novel Target for Tocolysis.

ABSTRACT:

Background

Spontaneous preterm labor leading to preterm birth is a significant obstetric problem leading to neonatal morbidity and mortality. Current tocolytics are not completely effective and novel targets may afford a therapeutic benefit.

Objective

To determine whether the anoctamin (ANO) family, including the calcium-activated chloride channel ANO1, is present in pregnant human uterine smooth muscle (USM) and whether pharmacological and genetic modulation of ANO1 modulates USM contraction.

Methods

Reverse transcription-polymerase chain reaction (RT-PCR), quantitative RT-PCR, and immunohistochemical staining were done to determine which members of the ANO family are expressed in human USM. Uterine smooth muscle strips were studied in an organ bath to determine whet

SUBMITTER: Danielsson J 

PROVIDER: S-EPMC6344949 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature