Single-cell analysis reveals congruence between kidney organoids and human fetal kidney.

ABSTRACT:

Background

Human kidney organoids hold promise for studying development, disease modelling and drug screening. However, the utility of stem cell-derived kidney tissues will depend on how faithfully these replicate normal fetal development at the level of cellular identity and complexity.

Methods

Here, we present an integrated analysis of single cell datasets from human kidney organoids and human fetal kidney to assess similarities and differences between the component cell types.

Results

Clusters in the combined dataset contained cells from both organoid and fetal kidney with transcriptional congruence for key stromal, endothelial and nephron cell type-specific markers. Organoid enriched neural, glial and muscle progenitor populations were also evident. Major transcri

SUBMITTER: Combes AN 

PROVIDER: S-EPMC6345028 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature