Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.

ABSTRACT: Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.

SUBMITTER: Strauss S 

PROVIDER: S-EPMC6345375 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature