Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva.

ABSTRACT:

Background

Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control campaigns. Previously, antibodies to sand fly saliva were detected using whole salivary gland homogenate (SGH) or recombinant proteins, both of which also have their disadvantages. This is the first study on sand flies where short peptides designed based on salivary antigens were successfully utilized for antibody screening.

Methodology/principal findings

Specific IgG was studied in hosts naturally exposed to Phlebotomus orientalis, the main vector of Leishmania donovani in East Afr

SUBMITTER: Sima M 

PROVIDER: S-EPMC6345433 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature