Avidin Adsorption to Silk Fibroin Films as a Facile Method for Functionalization.

ABSTRACT: Silk fibroin biomaterials are highly versatile in terms of materials formation and functionalization, with applications in tissue engineering and drug delivery, but necessitate modifications for optimized biological activity. Herein, a facile, avidin-based technique is developed to noncovalently functionalize silk materials with bioactive molecules. The ability to adsorb avidin to silk surfaces and subsequently couple biotinylated macromolecules via avidin-biotin interaction is described. This method better preserved functionality than standard covalent coupling techniques using carbodiimide cross-linking chemistry. The controlled release of avidin from the silk surface was demonstrated by altering the adsorption parameters. Application of this technique to culturing human foreskin fibrobl

SUBMITTER: Abbott A 

PROVIDER: S-EPMC6345528 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature