Filopodome Mapping Identifies p130Cas as a Mechanosensitive Regulator of Filopodia Stability.

ABSTRACT: Filopodia are adhesive cellular protrusions specialized in the detection of extracellular matrix (ECM)-derived cues. Although ECM engagement at focal adhesions is known to trigger the recruitment of hundreds of proteins ("adhesome") to fine-tune cellular behavior, the components of the filopodia adhesions remain undefined. Here, we performed a structured-illumination-microscopy-based screen to map the localization of 80 target proteins, linked to cell adhesion and migration, within myosin-X-induced filopodia. We demonstrate preferential enrichment of several adhesion proteins to either filopodia tips, filopodia shafts, or shaft subdomains, suggesting divergent, spatially restricted functions for these proteins. Moreover, proteins with phosphoinositide (PI) binding sites are particularly en

SUBMITTER: Jacquemet G 

PROVIDER: S-EPMC6345628 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature