Influence of accuracy, repeatability and detection probability in the reliability of species-specific eDNA based approaches.

ABSTRACT: Environmental DNA (eDNA) barcoding has a high potential to increase the cost-efficiency of species detection and monitoring in aquatic habitats. However, despite vast developments in the field, many published assays often lack detailed validation and there is little to no commonly (agreed upon) standardization of protocols. In this study, we evaluated the reliability of eDNA detection and quantification using published primers and assays targeting the Freshwater Pearl Mussel as a model organism. We first assessed limits of detection for two different target genes (COI and 16S) following the MIQE guidelines, and then tested the reliability of quantification in a double-blind mesocosm experiment. Our results reveal that different methodological indicators, namely accuracy, repeatability and

SUBMITTER: Mauvisseau Q 

PROVIDER: S-EPMC6345946 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature