High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry.

ABSTRACT: In life sciences, the material properties of suspended cells have attained significance close to that of fluorescent markers but with the advantage of label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded the application of mechanical cell assays to fast on-the-fly phenotyping of large sample sizes, but has been restricted to single material parameters as the Young's modulus. Here, we introduce dynamic real-time deformability cytometry for comprehensive cell rheological measurements at up to 100 cells per second. Utilizing Fourier decomposition, our microfluidic method is able to disentangle cell

SUBMITTER: Fregin B 

PROVIDER: S-EPMC6346011 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature