Complex-centric proteome profiling by SEC-SWATH-MS.

ABSTRACT: Proteins are major effectors and regulators of biological processes that can elicit multiple functions depending on their interaction with other proteins. The organization of proteins into macromolecular complexes and their quantitative distribution across these complexes is, therefore, of great biological and clinical significance. In this paper, we describe an integrated experimental and computational technique to quantify hundreds of protein complexes in a single operation. The method consists of size exclusion chromatography (SEC) to fractionate native protein complexes, SWATH/DIA mass spectrometry to precisely quantify the proteins in each SEC fraction, and the computational framework CCprofiler to detect and quantify protein complexes by error-controlled, complex-centric analy

SUBMITTER: Heusel M 

PROVIDER: S-EPMC6346213 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature