Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway.

ABSTRACT: Hepatic ischaemia-reperfusion (IR) injury is mainly attributed to a burst of reactive oxygen species (ROS) that attack biological macromolecules and lead to cell death. The superoxide anion (O₂˙^-) is the first ROS to be generated and triggers the production of other ROS; thus, explorations of the role of O₂˙^- in the IR process are meaningful. Meanwhile, the Golgi apparatus generates O₂˙^- via Golgi-associated proteins, which might play an essential role in IR injury. However, the molecular mechanism by which O₂˙^- from the Golgi apparatus regulates hepatic IR injury is unclear. Therefore, to solve this problem, a two-photon (TP) excited fluorescence probe (CCA) was designed and prepared for the rev

SUBMITTER: Zhang W 

PROVIDER: S-EPMC6346286 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature