Nucleotide and structural label identification in single RNA molecules with quantum tunneling spectroscopy.

ABSTRACT: Although a number of advances have been made in RNA sequencing and structural characterization, the lack of a method for directly determining the sequence and structure of single RNA molecules has limited our ability to probe heterogeneity in gene expression at the level of single cells. Here we present a method for direct nucleotide identification and structural label mapping of single RNA molecules via Quantum Molecular Sequencing (QMSeq). The method combines non-perturbative quantum tunneling spectroscopy to probe the molecular orbitals of ribonucleotides, new experimental biophysical parameters that fingerprint these molecular orbitals, and a machine learning classification algorithm to distinguish between the ribonucleotides. The algorithm uses tunneling spectroscopy measuremen

SUBMITTER: Abel GR 

PROVIDER: S-EPMC6346406 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature