Protein profiles of enzymatically isolated rumen epithelium in sheep fed a fibrous diet.

ABSTRACT:

Background

The rumen wall plays a major role in efficient transfer of digested nutrients in the rumen to peripheral tissues through the portal venous system. Some of these substrates are metabolised in the epithelium during this process. To identify the specific proteins involved in these processes, we used proteomic technologies. Protein extracts were prepared from ventral rumen tissue of six sheep fed a fibrous diet at 1.5× maintenance energy requirements. Using a newly developed method, we were able to enzymatically isolate the epithelial cells from underlying tissue layers, thus allowing cytosol and membrane fractions to be independently analysed using liquid chromatography tandem mass spectrometry (LC MS/MS).

Results

Using our procedure we identified 570 epithelial prot

SUBMITTER: Bond JJ 

PROVIDER: S-EPMC6346531 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature