CXCL12/CXCR4 promotes inflammation-driven colorectal cancer progression through activation of RhoA signaling by sponging miR-133a-3p.

ABSTRACT:

Background

Activation of CXCL12/CXCR4 axis has been found to be associated with invasion and metastasis in many cancers. However, the underlying mechanism remains elusive. Increasing data highlight that non-coding RNAs are linked to CRC progression.

Methods

The effects of CXCR4 were investigated using villin-CXCR4 transgenic mice model by flow cytometry assay, immunohistochemistry, and Western blot. The mechanism was explored through bioinformatics, luciferase reporter assay and RNA immunoprecipitation assay.

Results

We found that high CXCR4 expression exacerbated colitis-associated cancer in villin-CXCR4 transgenic mice. CXCR4^+/-Apc^min/+ compound mutant mice demonstrated higher colorectal tumorigenesis than Apc^min/+ mice. Furthermore,

SUBMITTER: Yu X 

PROVIDER: S-EPMC6346552 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature