Role of CsrA in stress responses and metabolism important for Salmonella virulence revealed by integrated transcriptomics.

ABSTRACT: To cause infection, Salmonella must survive and replicate in host niches that present dramatically different environmental conditions. This requires a flexible metabolism and physiology, responsive to conditions of the local milieu. The sequence specific RNA binding protein CsrA serves as a global regulator that governs gene expression required for pathogenicity, metabolism, biofilm formation, and motility in response to nutritional conditions. Its activity is determined by two noncoding small RNAs (sRNA), CsrB and CsrC, which sequester and antagonize this protein. Here, we used ribosome profiling and RNA-seq analysis to comprehensively examine the effects of CsrA on mRNA occupancy with ribosomes, a measure of translation, transcript stability, and the steady state levels of transcripts un

SUBMITTER: Potts AH 

PROVIDER: S-EPMC6347204 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature