Memory T cells targeting oncogenic mutations detected in peripheral blood of epithelial cancer patients.

ABSTRACT: T cells targeting shared oncogenic mutations can induce durable tumor regression in epithelial cancer patients. Such T cells can be detected in tumor infiltrating lymphocytes, but whether such cells can be detected in the peripheral blood of patients with the common metastatic epithelial cancer patients is unknown. Using a highly sensitive in vitro stimulation and cell enrichment of peripheral memory T cells from six metastatic cancer patients, we identified and isolated CD4⁺, and CD8⁺ memory T cells targeting the mutated KRAS^G12D and KRAS^G12V variants, respectively, in three patients. In an additional two metastatic colon cancer patients, we detected CD8⁺ neoantigen-specific cells targeting the mutated SMAD5 and MUC4 proteins. Therefo

SUBMITTER: Cafri G 

PROVIDER: S-EPMC6347629 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature