Molecular Characterization of Echinococcus granulosus Sensu Lato from Livestock in North Khorasan Province, Iran.

ABSTRACT:

Background

Echinococcus granulosus is one the most important zoonotic disease which is endemic in worldwide. Molecular method has allowed discrimination of different genotypes (G1-G10), providing new approach in development of prevention and control program of hydatid cyst. This study was conducted to identify the genotypes of E. granulosus from domestic animals in nine districts of North Khorasan Province using the mitochondrial cox1 gene sequence.

Methods

Overall, 122 hydatid cyst were collected during 2016-2017 from sheep (n=43) and cattle (n=79). DNA was extracted from protoscoleces and germinal layers and amplified by PCR. Phylogenetic analysis was also performed by analyzing the complete nucleotide sequences of mitochondrial cytochrome C oxidase subunit 1

SUBMITTER: Salehi M 

PROVIDER: S-EPMC6348223 | biostudies-literature | 2018 Oct-Dec

REPOSITORIES: biostudies-literature