FTO Knockout Causes Chromosome Instability and G2/M Arrest in Mouse GC-1 Cells.

ABSTRACT: N ⁶-methyladenosine (m⁶A) is the most abundant modification on eukaryotic mRNA. m⁶A plays important roles in the regulation of post-transcriptional RNA splicing, translation, and degradation. Increasing studies have uncovered the significance of m⁶A in various biological processes such as stem cell fate determination, carcinogenesis, adipogenesis, stress response, etc, which put forwards a novel conception called epitranscriptome. However, functions of the fat mass and obesity-associated protein (FTO), the first characterized m⁶A demethylase, in spermatogenesis remains obscure. Here we reported that depletion of FTO by CRISPR/Cas9 induces chromosome instability and G2/M arrest in mouse spermatogonia, which was partially rescued

SUBMITTER: Huang T 

PROVIDER: S-EPMC6348250 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature