5-Oxyacetic Acid Modification Destabilizes Double Helical Stem Structures and Favors Anionic Watson-Crick like cmo⁵ U-G Base Pairs.

ABSTRACT: Watson-Crick like G-U mismatches with tautomeric G^enol or U^enol bases can evade fidelity checkpoints and thereby contribute to translational errors. The 5-oxyacetic acid uridine (cmo⁵ U) modification is a base modification at the wobble position on tRNAs and is presumed to expand the decoding capability of tRNA at this position by forming Watson-Crick like cmo⁵ U^enol -G mismatches. A detailed investigation on the influence of the cmo⁵ U modification on structural and dynamic features of RNA was carried out by using solution NMR spectroscopy and UV melting curve analysis. The introduction of a stable isotope labeled variant of the cmo⁵ U modifier allowed the application of relaxation dispersion NMR to probe the pot

SUBMITTER: Strebitzer E 

PROVIDER: S-EPMC6348377 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature